Assessment of Anxiolytic Activity of Brahmi (<i>Bacopa monnieri</i>) in Zebrafish Model System

The increasing prevalence of anxiety and stress-related disorders has made it a leading contributor to the global health burden. present treatment options have severe side effects show remission on discontinuation medication. Hence, there is an urgent need explore safer alternative treatments for long-term usage with minimum toxicity. medicinal plant Brahmi (Bacopa monnieri) been used in Indian traditional medicine as neural tonic centuries. study aimed toxicity anxiolytic activity using zebrafish model system. assays determined effective concentration be 0.01%. In addition, behavioral such thigmotaxis scototaxis endocrine measurement cortisol levels stressed larvae were performed. Zebrafish embryos exposed 0.2% up seven days post fertilization (dpf) did not any developmental Behavioral performed 5dpf treated 0.01% extract. Our studies that significantly reduced (wall hugging) behavior osmotic stress compared untreated larvae. Stress activates hypothalamic-pituitary-interrenal axis stimulates release Whole body assay shown stress-induced report mitigates response This suggests may safe option long term management stress.